Journal: International Dental Journal

Article Title: FAM30A Induces Inflammation and Oxidative Damage in PDLSCs by Targeting miR-424-5p

doi: 10.1016/j.identj.2026.109605

Figure Lengend Snippet: Effects of si- FAM30A on LPS-induced PDLSCs function and inflammatory levels. ( A) Exploration of optimal LPS concentration for inducing PDLSCs. ( B) Establishment of FAM30A knockdown cell lines. ( C) Effects of si- FAM30A transfection on PDLSCs proliferation. ( D) Apoptotic changes in PDLSCs following FAM30A inhibition. ( E) Expression of inflammatory-associated factors (TNF-α, IL-1β, IL-10) in PDLSCs following si- FAM30A transfection. ( F-H) Levels of oxidative stress markers malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in PDLSCs after FAM30A inhibition. * P < .05 , ** P < .01 , *** P < .001 , **** P < .0001 .

Article Snippet: PDLSCs were induced for 12 hours using 100 ng/mL lipopolysaccharide from E. coli O55:B5 (LPS, HY-D1056, MCE, U.S.) to establish an in vitro cellular model.

Techniques: Concentration Assay, Knockdown, Transfection, Inhibition, Expressing

Journal: iScience

Article Title: ZDHHC17-mediated IKKβ palmitoylation suppresses NF-κB signaling to mitigate the progression of liver fibrosis

doi: 10.1016/j.isci.2026.116360

Figure Lengend Snippet: ZDHHC17 deficiency stimulates pro-inflammatory cytokines production in macrophages leading to enhanced expression of fibro-genic genes in HSCs (A) THP-1 cells were induced to M0 macrophages by PMA and further polarized to M1 macrophages by treatment with LPS/IFN-γ. ZDHHC17 was over-expressed or knocked down in M1 macrophages by plasmid transfection or siRNA. Cells were collected 48 h post-transfection for detection. LX2 cells were seeded onto the lower chamber, and M1 macrophages were plated on the upper chamber of a 0.4 μm transwell membrane, which prevents direct cell contact, limiting cell interaction to secretory chemicals. (B and C) ZDHHC17 was significantly overexpressed in M1 macrophages by plasmid transfection (B) and decreased by small interfering RNAs (siRNAs) (C). Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of the pro-inflammatory cytokines (TNF-α, IL-1β, and IL-12β) were detected in ZDHHC17-overexpressed (D) or ZDHHC17-silenced (E) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). ELISA detected serum TNF-α, IL-1β, and IL-12β in ZDHHC17-overexpressed (F) or ZDHHC17-silenced (G) macrophages. Data are presented as the mean ± SD ( n = 6 independent experiments). mRNA expression of α-SMA, COL1A1, TGF-β, and TIMP-1 was detected by qPCR in LX-2 cells following co-cultured with ZDHHC17-overexpressed (H) or silenced (I) macrophages. Data were presented as mean ± SD ( n = 6 independent experiments). ∗ p < 0.05 , ∗∗ p < 0.01 , ∗∗∗ p < 0.001 , ∗∗∗∗ p < 0.0001 by Student’s t test (B-I).

Article Snippet: LPS (Lipopolysaccharide) , MCE (Monmouth Junction, NJ, USA) , Cat# HY-D1056.

Techniques: Expressing, Plasmid Preparation, Transfection, Membrane, Enzyme-linked Immunosorbent Assay, Cell Culture